Generator
Part:BBa_K1741013:Design
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
sfGFP under proC promoter with a hairpin in 5'UTR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 232
Design Notes
A weak hairpin introduced to 5'UTR.
Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.